Journal: Heliyon
Article Title: Klebsiella pneumoniae -OMVs activate death-signaling pathways in Human Bronchial Epithelial Host Cells (BEAS-2B)
doi: 10.1016/j.heliyon.2024.e29017
Figure Lengend Snippet: Detection of apoptosis by TUNEL assay. The cell monolayer exposed to K. pneumoniae -OMV (50 μg) and LPS (50 μg) for 14 h was fixed with 4 % paraformaldehyde and permeabilized with 0.25 % Triton-X 100. DNA fragmentation was detected through labeling of the 3′-OH terminals with EdUTP, mediated by the TdT enzyme. Images were acquired using the TCS SP5 laser scanning confocal microscope at 20 × magnification, with a 2.5× zoom of a representative field. The unexposed cells (CTRL negative) showed regular nuclei and uniform blue staining of the chromatin, while the cells treated with purified vesicles and LPS were TUNEL positive, recording an intense red staining. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Article Snippet: TEM images were acquired using an EM 208 S transmission electron microscope (Philips, Amsterdam, Netherlands).
Techniques: TUNEL Assay, Labeling, Microscopy, Staining, Purification